Yue-Ling Zhang is a PI in Guangdong Provincial Key Laboratory of Marine Biotechnology, School of Sciences, Shantou University. He received his PhD from Xiamen University, China and studied at Uppsala University, Sweden as a visiting scholar. He was a Professor and Group Leader of Department of Biology, Shantou University, China from 11/2011 to 03/2013, 05/2014 to 11/2016, and then moved to Marine Biology Institute, Shantou University, China as a Professor and Group Leader since 11/2016. He is the Director of Guangdong Province Society for Biochemistry and Molecular Biology, a Standing Director of Guangdong Province Society for Biochemistry and Molecular Biology, a Director of Guangdong Province Society for Zoology, and a Director of Guangdong Province Society for Aquaculture. His main research is focusing on shrimp immunology, especially the immune mechanisms of crustacean hemocyanin. So far, he has been the recipient of about 20 foundations as Principal Investigator. In peer-reviewed publications, his group has published 46 research papers in J Proteome Res, Fish Shellfish Immunol, FEBS Lett, Dev Comp Immunol, Immunol Lett, PloS ONE , Mol Immunol, J Shellfish Res and so on.
Hemocyanin (HMC) is a multifunctional protein which plays many essential roles in invertebrate organisms. Recently more and more immune-related functions have been discovered on this protein. Here, the shrimp was infected with Vibrio parahaemolyticus and the shrimp sera were analyzed by two-dimensional gel electrophoresis. Totally 15 spots were identified as significantly up-regulated spots and further analyzed by MALDI-TOF/TOF mass spectrometry. Four of them were identified as HMC derived truncations (HMCS1, HMCS3, HMCS4 and HMCS5). The HMCS4 primary sequence was further determined via Edman N terminal sequencing, MALDI-TOF MS and amino acid sequence alignment. The result indicated that the HMCS4 was a 165aa fragment from shrimp HMC small subunit C-terminal. The HMCS4 immunological activities were further analyzed by agglutination experiment and anti-bacterial assay in vitro. The results showed that the recombinant HMCS4 had strong agglutination and antibacterial activities against pathogenic bacteria at the optimum bacteriostasis concentration. In addition, the HMCS4 immunological activities were explored via mortality assay in vivo. The shrimp was challenged with V. parahaemolyticus and HMCS4 V. parahaemolyticus mixture separately. The shrimp mortality rate significantly decreased at 96 h post-infection with HMCS4 injection. Our data showed that shrimp HMC truncation generation upon infection was an effective immune response against invaded pathogens. Moreover, these findings may have some potential applications in shrimp industry.
Carolina Alonso Pozas has completed her graduation in Marine Sciences from the University of Cadiz. In addition to collaborating in various research projects at the Spanish Institute of Oceanography she complemented his training with the completion of a Master’s in Sustainable Management in Marine Systems and a Master’s in Integrated Quality Management. In close connection with the Fisheries sector, she has been a Fishery Observer in vessels of Newfoundland waters (Canada) in support of the control of the Community Fisheries Policy. Since 2005, she has been working in Fisheries and Aquaculture for Andalusian Government in Spain.
TASCMAR is a collaborative research project funded under the EU Horizon 2020 programme and aspires to develop new tools and strategies to overcome existing bottlenecks in the discovery and application of marine-derived biomolecules, with a focus on the theme of anti-ageing. Marine biotechnology remains an emerging field involving the discovery and the application of products and processes derived from marine organisms. In comparison to terrestrial natural products (NPs), marine organisms possess the capacity to produce a huge diversity of molecules with unique structural features and biological potency. On the other hand, ageing of the population has become a worldwide demographic trend. According to WHO by 2040, the global population aged 65 and over is estimated to reach 1.3 billion (14%) of the total population and, consequently, the prevalence of age-related diseases (e.g. cardiovascular diseases, metabolic disorders, neurodegeneration, cancer, etc.) will significantly increase. Taking into consideration the aforementioned issues, the TASCMAR project aspires to develop new tools and strategies in order to overcome existing bottlenecks in the biodiscovery and industrial exploitation of novel marine derived biomolecules (secondary metabolites and enzymes) with applications in the pharmaceuticals, nutraceuticals, cosmeceutical and fine chemicals industry. Exploitation of neglected and underutilized marine invertebrates and symbionts from the under-investigated mesophotic zone, of existing and targeted new collections from global marine biodiversity hotpots will be combined with innovative approaches for the cultivation and extraction of marine organisms from lab to pilot-scale. The activities in the project will be continuously evaluated for their socio-economic and environmental impacts to reach a compromise between industrial development and sustainable growth.