Day 3 :
- Track 1: Aquatic Farming Methods: Hatchery, Nutrition and Feed Management
Track2: Fish Habitat, Ecology and Conservation
Track 3: Comparative Biology of Aquatic Species
Track 4: Aquaculture Related Diseases and Health Management
Track 5: Aquaponics- Planning, Construction and Maintainance
Session Introduction
Helmut Wedekind
Bavarian States Research Center for Agriculture, Germany
Title: Recirculating aquaculture – Status and developments
Biography:
Helmut Wedekind has completed his PhD at the age of thirty one years from Göttingen University on postdoctoral studies on intensive catfish farming. He is the director of the Institute for Fisheries, part of the Bavarian States Research Center for Agriculture in Starnberg, which is a research and education center for fisheries and aquaculture. He has published papers in reputed journals and in national fisheries and aquaculture journals. Moreover he is a lecturer at the Technical University of Munich and other German high schools in the field of aquaculture
Abstract:
The cultivation of different aquatic organisms in recirculating aquaculture systems (RAS) is practiced since decades in Europe, the USA and other countries worldwide. Due to different limitations of open aquaculture systems (e. g. ponds or net cages), and restrictive regulations of water supplies and waste, water reuse is becoming more and more relevant. Whereas heated RAS in isolated buildings were already established in the eighties, the increasing use of the recirc-technology in cold water aquaculture (e. g. salmonid farming) is a relatively new development. When compared to traditional aquaculture, both - warm water RAS and cold water RAS - are characterized by a comparatively sophisticated technology and high operating efford and capital costs. Consequently, mangers of RAS are in need for a thoroughly base of specific know-how. For successful RAS farming fundamental knowledge in the field of biology, production technology, mechanical and biological filtration, stock management, feeding, health management, controlling, biosecurity, and economics is essential. All this has to be considered for warm water RAS, which accomplish high recirculation rates, and cold water RAS with a higher daily water exchange. Actually, the production of different tropical fish and crustaceans, as well as species cultured under temperate temperature – including freshwater and marine organisms – recently is rapidly developing
Bernhard Feneis
German Aquaculture Association, Germany
Title: Fish diseases and treatment in the light of EU Law
Biography:
Bernhard Feneis studied Fishgenetic on Technical University of Munich and as a second fulltime study course Veterinary Medicine on Ludwig Maximilian University Munich . working as fish Vet at bavarian Fish health service and on privat bases in EU Memberstates. He is Vice President of FEAP, the Federation of European Aquaculture Producers, and President of the German Aquaculture Association VDBA
Abstract:
A overview of freshwater fish diseases is presented, divided in viral, bacterial and parasite aetiology. The different disease patterns are described, underlined with slides showing typical symptoms.The relevant EU regulations, like directives and laws within the EUanimal/human health philosophy is presented and will be discussed. Following core topics are: The directive 88/2006, including five Categories of disease free level. Definition and handling of exotic and nonexotic notifiable diseases. The national implementation of the framework, differences between memberstates “Traces”, a method to prevent spreading diseases by transporting fish to member states, including a flowchart for administration as well as for fishfarmers. A special part will discuss the situation of “new” diseases, not yet in the focus of the framework, where partly is even not yet a standardized diagnostic or treatment available. E.g. CEV, Sleeping disease trout, strawberry disease. What will change when the new Animal Health Law (AHL) comes into force? The necessity of vaccines within a biosecurityplan instead of Antibiotic use is discussed especially in the light of the new EU legislation. What changes in the cascade of the new AHL , when you need a drug for a treatment when there is nothing direct meant for the disease in the relevant fish species. In the final part the problems of fishfarmers using the framework TRACES and RL88&2006 will be discussed, and the demands are formulated to have a competitive basis on the european- and worldmarket.
Biography:
Guluzar Tuna Kelestemur has completed her PhD at the age of 30 from Firat University, Faculty of Fisheries, Elazig/TURKEY. Her expertise areas are fishfeeding, stress in fish. She had published more than 20 papers in reputed journals, reviewer in different journals and have been serving as an editorial board member of repute.
Abstract:
The present study investigated the effects of propolisand vitamin E supplementation in diets of juvenile rainbow trout subjected to two different flowrates (0.9and 2.1 L min−1) on growth performance, and vitamin A, C and E concentrations in tissues as well as malondialdehyde (MDA) levels. Juvenilerainbowtroutwere fed withdietscontaining 10 and 30 g propolis kg−1, 60 mg kg−1 vitamin E (Rovimix E-50 adsorbate; min.%50 dl-α-tokopherlyacetate) andwithoutsupplementedbasaldietfor 12 weeks. Weightgain (WG) in the C groupwassignificantlylower (P < 0.05) than P10, P30 and E60 groups at bothflow rate treatments. At 2.1 L min−1, specificgrowth rate (SGR) in the C group was significantly lower (P < 0.05) than other groups, but at 0.9 L min−1, SGR of fish didnot differ among thedietsgroups (P > 0.05). Survival rate (SUR) was higher in propolis and vitamin E supplemented dietgroups (P < 0.05) than control diet group at 0.9 L min−1. Fish fed on diet E60 had higher (P < 0.05) tissue vitamin E concentration than fishes fed on other diets groups. Vitamin C concentration in rainbowtrout tissues was significantly affected by the 30 g propolis supplemented diet group (P < 0.05), followed bythe 10 g propolis supplementeddiet group (P < 0.05). MDA level of E60 group was found significantly decreased instead of different than other groups (P < 0.05). The results of Student's t-test revealed that WG, SGR, SUR values, vitamin (A, C, E) concentrations and MDA levels of tissues were negativelyaffected by0.9 L min−1flow rate treatment in juvenile rainbowtrout.
Rossita Shapawi
Universiti Malaysia Sabah, Malaysia
Title: The potentail of crude palm oil as a dietary lipid source in the practical diets of hybrid grouper Epinephelus fuscoguttatus x Epinephelus lanceolatus
Biography:
Rossita Shapawi is currently working in Borneo Marine Research Institute, Universiti Malaysia Sabah, Malaysia
Abstract:
The present study was carried out to evaluate the long termfeeding (4 months)of crude palm oil-based (CPO) diets to hybrid grouper (Epinephelus fuscoguttatus x Epinephelus lanceolatus). Five isolipidic (16%) and isoproteic (50%) practical diets were formulated to contain differentreplacement level of fish oil with crude palm oil. A control diet was supplemented with 100% fish oil labelled as FO and the rest of the experimental diets were labelled as 25CPO, 50CPO, 75CPO and 100CPO indicating 25, 40, 75 and 100% replacement of fish oil with CPO.The initial fish (approximately 200 g) were randomly distributed into groups of 30 fish in square cages (2.0 m depth and 1.5 m diameter) in the sea. The triplicate groups of hybrid grouper were fed once a day at apparent satiation level. Growth measurement was carried out on a monthly basis.At the end of the feeding trial, fish fillet were subjected to sensory evaluation analysis. No significant differences (p>0.05) in terms of growth among fish fed with various experimental diets with weight gain and SGR ranging from 171.43 % (75CPO) - 181.63 % (50CPO) and 0.83±0.01-0.86±0.02%/d, respectively.Survival rate of fish were considered very high during this 4 months feeding trial with values above 93%.Based on the 5-point hedonic scale of acceptance test, all fillets were neither like nor disliked by the panelists.There is no particular trend of acceptance in all attributes among the fish fillets implying fillets of fish fed with CPO are well received by the consumers.
Ozlem Emir Coban
Firat University, Turkey
Title: Evaluation of essential oils as a glazing material for frozen rainbow trout Oncorhynchus mykiss fillet
Biography:
Ozlem Emir Coban is currently working as Associate Professor at Faculty of Fisheries,Turkey. She is serving as reviewer in more than 15 international journals and editorial board member in more than 12 scientific international journals. She is EDITOR IN CHIEF of International Journal of Oceanography and Marine Ecological System.She also received certificates for Basic Quality Management, Environmental Management, occupational Safety and Health, Internal Auditor, Risk Analysis and Food Safety.
Abstract:
In this study, sage, thyme and clove oils were evaluated as a glazing material for rainbow trout (Oncorhynchus mykiss) frozen. One percent sage, thyme and clove oil solutions were used for glazing. Water-glazed (WG) and nonglazed (NG) rainbow trout fillets were used as controls. Fish fillets were glazed and were stored in a freezer at -18C for 6 months. Samples were analyzed at 1, 2, 3, 4, 5 and 6 months for moisture content, total volatile basic nitrogen, thiobarbituric acid, peroxide value, free fatty acids and sensorial quality. It was determined that glazed samples had higher moisture content compared to NG trout fillet after 6 months storage. Essential oils were effective in controlling lipid oxidation in rainbow trout fillet (P < 0.05).
Luiz Fernando Loureiro Fernandes
Universidade Federal do EspÃrito Santo, Brazil
Title: The struggle to convince traditional fishermen to become aquaculturists in Southeast Brazil
Biography:
Abstract:
Jiashou Liu
Chinese Academy of Sciences, China
Title: Culture-based fisheries based on biomanipualtion bring about better economic benefits and biodiversity recovery in lakes of China
Biography:
Jiashou Liu is currently professor in State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, China
Abstract:
Lakes are important for stock enhancement fisheries in China. More than 10 species are stocked into lakes to increase production. The most common species stocked are planktivorous silver carp, bighead carp, and herbivorous grass carp. However, stocking of silver carp and bighead carp has resulted in eutrophication because of manure application. Stocking of grass carp has resulted in the disappearance of macrophytes. In this paper, two case studies in two lakes of the Yangtze River basin in China are introduced. One case is the replacement of low valued carps by high valued mandarin fish and Chinese mitten crab, resulting in better economic benefits and water quality in Wuhu Lake, Hubei Province. The stocking rate of mandarin fish is determined by food consumption rates, which is mainly related to water temperature and fish size, and prey fish productivity, which is mainly related to mean biomass of prey fish, production/biomass ratio and temperature. The bioenergetics model of mandarin fish is established to predict the growth and consumption of prey fish in stocked lakes. The stocking model of mitten crab in culture-based fisheries is also established based on biomass of macrophyte coverage, benthos biomass and ratio of Secchi depth to mean water depth in lakes. The other case is stock enhancement of combined piscivorous fishes based on fish community structure, resulting in better water quality and recovery of macrophyte biodiversity in Kuilei Lake, Jiangsu Province.
Jun Li
Chinese Academy of Sciences, China
Title: Physiological and immune responses of Atlantic salmon after high stocking density stress in RAS
Biography:
Jun Li has completed his PhD at the age of 33 years from Institute of Oceanology, Chinese Academy of Sciences. He is the director of Marine Fish Aquaculture and Biotechnology Research Group. He has published more than 100 papers in reputed journals and has been serving as an editorial board member of repute
Abstract:
In order to investigate the effects of stocking density stress on the physiological and immune responses of Atlantic salmon in RAS, five stocking densities (6, 9,15,21 and 24 kg/m3) groups of Atlantic salmon (180D, 1-1.5kg) were reared in five square concrete ponds (90m3). The mortality was obviously affected by stocking density and the content of DO decreased following the density increased, NH3/NH4- showed a positive correlation with density of CO2 content. The specific growth rate, final weight and weight gain in the HSD group were significantly lower than those in the LSD and MSD groups. T3 and GH showed significant decrease with the increase of the stocking density. The content of cortisol in HSD group was significantly differentiated from MSD and LSD groups after 40 days, and then the content among the five groups were nearly equal after 130 days. Our results also showed that the levels of leucocyte numbers and hemoglobin expressions were positively correlated with stocking density after 100 days. The level of lysozyme expression in liver and kidney of high density group began to decline and rise since 130 and 190 days, respectively. GOT activities showed no significance before70 days, and group A increased quickly after 100 days. The level of serum GPT activities showed the similar changes with GOT. According the our results in the this study, the stocking density of 30 kg/m3 was considered as the node in RAS caused by density stress.
Zhongjie Li
Chinese Academy of Sciences, China
Title: Aquaculture in China: Achievements, challenges and perstectives
Biography:
Zhongjie Li is currently professor in State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of Sciences, China
Abstract:
It is acknowledged that China is the mainstay in global aquaculture, contributing for example 65.7 (of 76,321,310 t) and 63.6 (of 38,994,913 t) percent to total global and freshwater aquaculture production, respectively in 2011, significantly increasing the corresponding contributions from36.1 and 38.5 percent of the global total (7,359,881 t) and global freshwater (2,342,706 t) aquaculture production in 1989. Overall,aquaculture production in China in turn has enabled to reduce our dependence on food fish supplies from a hunted to a farmed origin, like all the other staples. It is evident that though inland aquaculture is practiced in most provinces of China, the great bulk of it occurs in the area that lies approximately between 110 to 120 °E and 19 to 35 °N. Importantly, the provinces in the Yangtze River Basin accounted for 66 percent of the inland aquaculture production in China.The major changes that are likely to occur in freshwater aquaculture in Yangtze River Basin in all probability will be associated with minimizing environmental perturbations arising from aquaculture practices. In regard to the trends of freshwater aquaculture in Yangtze River Basin, two aspects can be expected. Firstly, it is the stricter use of natural freshwaters including lakes, reservoirs and rivers for aquaculture.Secondly, in compensation for aquaculture production losses from natural water bodies, more attention will be paid to controlled water bodies
Iman Abumourad
National Research Centre, Eygpt
Title: Disease hazards of petroleum hydrocarbons contamination in Gafrarium pectinatum (LINNAEUS, 1758) from Egyptian lake Temsah
Biography:
Iman abumourad is professor of fish Molecular Biology, National Research Centre, Egypt. She has completed here PhD from Menoufia University, Egypt and postdoctoral studies from Bordoux 1 University, France and hydrobiologu institute, China. She has published more than 28 papers in reputed journals and has been serving as an editorial board member in reputed journals
Abstract:
Assessment of petroleum Hydrocarbons (PHC) contamination was carried out along with the water samples of Egyptian Lake Temsah, Suez Canal. Disease risk probability due to PHC contamination using cytochrome p4501A1 and glutathione S-transferase genes expression by RT-PCR in Gafrarium pectinatum bivalve in association with the investigation of parasitic infection were applied. Results indicated 0.85% µg/l petroleum Hydrocarbons contamination of Lake Temsah waters. Obvious increase in the cytochrome p4501A1 and glutathione S-transferase mRNA level was observed in response to Hydrocarbons contamination. 60% Marteilia refringens parasite infection was reported which may be correlated to PHC contamination.This study provides a basis for studying hydrocarbon detoxification processes in marine bivalves, especially Gafrarium pectinatum
Biography:
Tipsuda Thongbuakaew has completed her PhD at the age of 30 years from Mahidol University. She is the lecturer of School of Medicine, Walailak University. She has published 3 papers in reputed international journals
Abstract:
We demonstrated for the first time the existence and distribution of kisspeptin-like peptides (Kiss-l) and their receptors in the central nervous system (CNS) of the giant freshwater prawn, Macrobrachium rosenbergii, and that these peptides could induce the oogonial proliferation, ovarian maturation, and spawning. Using immunohistochemistry, kisspeptin1-like-signal (Kiss1-l signal) was detected in the neurons of clusters 6, 11 and 14/15 and also in olfactory neuropil (ON). Kisspeptin2-like-signal (Kiss2-l signal) was observed in clusters 6, 9, 10, 16 and 17 as well as in the anterior and posterior median protocerebral neuropils (AMPN/PMPN), ON, central body (CB) and protocerebral bridge (PB) and olfactory globular tract (OGT). In the subesophageal (SEG) and thoracic ganglia (TG), both Kiss1-l and Kiss2-l signals were detected in some neurons of dorsolateral cluster (DLC), ventromedial cluster (VMC) and also in fibers of associated neuropils. In abdominal ganglia (AG), Kiss1-l signal was detected in some neuronal clusters, whereas Kiss2-l signal was detected only in fibers of the AG. Functional bioassays demonstrated that both Kiss1- and Kiss2-treated prawns showed significantly shortened ovarian cycle (16±1.63 and 22.86±1.07 days) compared with the control (36±2 days). Moreover, both Kisses significantly increased the numbers of oogonial proliferation and spawned eggs, while the percentages of fertilized eggs in all treated groups were not significantly different from the control. These findings suggest that Kisses are highly conserved peptides that may also exist in the prawns and control their oogonial proliferation and ovarian maturation.
Yong Li
Chinese Academy of Sciences, China
Title: Research and application of aquatic animal eco-nutrition based on industrialized culture
Biography:
Yong Li has completed his PhD from China Agricultural University in 2001. He is a professor of Institute of Oceanology, Chinese Academy of Sciences. His research interests is ecological nutrition and clean feed on aquatic animal. At present, he acts as a principal of projects on aquatic animal nutrition and feed research, and pay attention to theory study and technical development on ecological nutrition and environmental stabilization for aquaculture. He is the standing director of Qingdao Society of Fisheries and Qingdao Innovation Union of Feed. He also is the director of Academic Committee, Jiangsu Province Innovation Union of Mariculture
Abstract:
The authors were the first to define the aquatic animal eco-nutrition and feed with nutrition emission reduction in order to reduce the negative effects of water environment and health, those caused by nutrition, feed and feeding. The researches systematically regulated internal and external environment of aquatic animals, guided by new theory and method of eco-nutrition and also adopted modern nutrition, biotechnology, processing technology, feeding strategy, etc...We obtained 6 important achievements after 10 years experiment study. “Rhomb Characteristics” was firstly named and defined that the relationship between WGR and NH4+-N was typical of protein eco-nutrition and the change curve looked like a rhomb. Achieved eco-nutrition requirements of main nutrients for some marine fish in recirculation aquaculture system (RAS), providing important parameters for developing ecological and valuable compound feed. Preliminary ascertained the effects of diet protein, satiety degree on growth, N excretion, digestion and immunity of Cynoglossus semilaevis G. Produced initial success on association study between eco-nutrition and molecule nutrition. The development on compound feed with N and P emission reduction for flatfish made a breakthrough and the new products applied rapidly. Scored important result on study of feeding strategy and intake model. In conclusion, the results provided theoretical basis and applicable technology for the goal of sustainable aquaculture that is the balance and harmonious development among suitable nutrients, stability environment, healthy growth, excellent products and cheap cost.
Wilma Q Chua
Cagayan State University, Philippines
Title: The effect of preservatives on the keeping quality of spiced dried tuna sticks (Katsuwonus pelamis)
Biography:
Wilma Q Chua is currently professor in Cagayan State University, Philippines
Abstract:
This study was conducted at the College of Fisheries Fish Processing Laboratory, Cagayan State University, Aparri, Cagayan from August to October 2003. It aimed to determine the effect of preservatives (citric and sobic acids) on the shelf-life of spiced-dried fish sticks wrapped with waxed paper. It was limited to the use of skipjack tuna (Katsuwonus pelamis). Sensory and physical evaluation on color, taste, and moisture content changes of the product were done. Microbiological examination (mold count) of the product was also undertaken. Results of the study revealed that products treated with .02% sorbic acid had the longest shelf-life at ambient temperature which was 35 days. The product showed lower moisture absorption and lower mold count as the storage days progressed. Molds that grew on the product were identified to belong to the genera Mocor aspergillus and Rhizopus.
Xian Li
Chinese Academy of Sciences, China
Title: Colour preferences of juvenile turbot (Scophthalmus maximus)
Biography:
Xian Li has completed his PhD at the age of 26 years from Institute of Oceanology, Chinese Academy of Sciences. She has published more than 16 papers in reputed journals
Abstract:
The background colour of aquaculture tanks was ordinarily chosen according to practical experience and/or observations of the fish behaviour and growth rates achieved. However, some farmed species, such as fish, are sentient and can make choice, showing preferences to environments. In the current study, a self-referent colour preference device was developed and the self-referent colour preference of farmed fish investigated. In experiment 1, the background colour preference of juvenile turbot cultured under a grey background for >3 months post-incubation was evaluated. Based on these results, in experiment 2, juvenile turbot were adapted to blue, pink, white and black backgrounds for 50 days and their preferences established. Meanwhile the growth rates, feed intake and the metabolic rates (including oxygen consumption rate and ammonia excretion rate) were evaluated. We found that turbot farmed under a grey background, or after long-term white, blue, pink and black colour adaptation, always displayed a preference for a white background and a dislike for black, red, or brown backgrounds, although their body colour was greyish. Long-term adaptation influenced the frequency of juveniles selecting white, black, pink or blue backgrounds. They showed the highest growth rate, feed intake and the metabolic rates under the blue and white colours, and the lowest under the black colour in accordance with their preferences. While how turbot made their decisions on self-referent colour preferences in a short time needs more investigations. These results indicate that dark colour is not suitable for turbot culture in view
Biography:
Mossamat Rashida Akter is currently working as visiting scholar in James Cook University, Australia
Abstract:
The aim of the present study was to assess the current status and future potential of the fresh water and marine aquaculture industries in Far North Queensland 2015 using semi-structured interviews and administered questionnaires to elicit information from aquaculturalists in the region (n= 5 comprising 4 barramundi farmers and 1 Queensland grouper farmer).The results revealed that despite aquaculture undergoing rapid growth and transformation around the world, the industry in Far North Queensland has a long way to go to reach its full potential and to compete with other countries in the region in terms of technology and in updating government policy and legislative frameworks to facilitate the expansion of sustainable aquaculture. Those interviewed stated that here have been no applications for new aquaculture farms for last 15 years in Far North Queensland, and that the lack of development may be attributed to the following factors: the regulatory burden is high; the standards imposed by regulators do not reflect the true impact of the industry and are in need of review; that the industry has difficulty attracting, training and maintaining appropriately qualified labour; and, that the industry needs to modernise and diversify. Nonetheless, most of those interviewed are optimistic about the future of their industry, provided these matters are urgently and constructively addressed.
- Track 6: RAS- Recirculating Aquaculture Systems
Track 7: Aquatic Biology- Immunology, Physiology, Toxicology
Track 8: Diversification in Aquaculture
Track 9: Aquaculture Environment Interactions- Measuring, Modelling and Mitigation
Track 10: Aquaculture Law and Policy
Session Introduction
Zainal Abidin Muchlisin
Syiah Kuala University, Indonesia
Title: Length weight relationships and condition factor of snapper (Lutjanus russelli), pony fish pepetek (Leiognathus fasciatus) and white-spotted spinefoot (Siganus canaliculatus) harvested from Ulelhee Bay, Banda Aceh City, Indonesia
Biography:
Zainal Abidin Muchlisin is a Professor in Ichthyology. He was graduated in Aquaculture from University of Riau, Indonesia (Bachelor in Aquaculture). He started working for Syiah Kuala University from 1999 to present. Muchlisin was completed his M.Sc & Ph.D Degree from Universiti Sains Malaysia in Aquaculture & Ichthyology, respectively. He has published 53 papers in reputable international journal which is indexed by Scopus and Thomson Reuter. Prof. Muchlisin is the editor for several journals, proceedings and books & reviewer for several referred international journals
Abstract:
The objective of present study was to examine the length-weight relationships and condition factor of three species fishes harvested from Ulelhee Bay, Aceh Province, Indonesia. The sampling was conducted during three month between on April until June 2015 using beach seine in Ujung Pancu Waters, Ulelhee Bay, Indonesia. The length weight relationship was calculated using Linear Allometric Model (LAM) and the relative weight was performed to assess the condition factor of the fish samples. A total of 900 fish samples (300 individual for every species) were measured for the length weight relationships and condition factor. The results showed that snapper (Lutjanusrusselli) has the b value of 3.04, indicates isometric growth pattern, while ponyfish (Leiognathus fasciatus) and white-spotted spinefootfish (Siganuscanaliculatus) have the b values of 1.41 and 1.75, respectively. These species display the allometric growth pattern. The relative weight condition factor ranges from 58.73 to 166.09 for L. russelli (101,52 in average), from 70.55 to 129.65 (100.72 in average) for L. fasciatus, and from 71.63 to 133.53 (100.59 in average) for S. canaliculatus. In general, the relative weight condition factors tended to 100, indicate the Ulelhee Bay is still in good condition and support the fish to growing well
Sunil Narayan Khade
Phulsing Naik College, India
Title: SCUBA diving and diversity of edible bivalve and gastropod molluscs from Raigad, Ratnagiri, Maharashtra, West Coast of India
Biography:
Sunil Narayan Khade has completed Ph.D. at the age of 33 from Dr. Babasaheb Ambedkar Marathwada, University, Aurangabad. He is the Assistant Professor in Phulsing Naik, College, Pusad. He has published more than 4 papers in reputed journals. He has completed courses in SCUBA like Snorkeling, Advanced Open Water, Emergency First Responder, Rescue Diver, and Dive Master
Abstract:
Diversity of bivalve and Gastropod molluscs was studied twice in each season monsoon, post monsoon, winter and summer July 2012 to June 2013. At each locality diversity and number of species were collected from Kundalika river (creek), Bhatye estuary, Shirgaon creek, Mirya, Bhawati-bander and local markets. The total numbers of molluscs was comprised of species namely Perna viridis, Marcia opima, Paphia malabarica, Meretrix meretrix, Gafrarium divaricatum, Tellina sinuata, Crassostrea catuckensis, Saccostrea cucullata, Sangvinolaria acuminata, Arca granosa, Polymesoda maxima, Dosina prostate,. Total number of 32 bivalve species while 89 species of gastropoda identified during study period. For the open sea used SCUBA Diving by DM. From these study localities is a wide chance of research to further explore both on the possibility of commercial value and ecosystem conservation.
Esther Michelle Gunben
Polytechnic of Sandakan, Malaysia
Title: Preliminary Study: Effect of feeding frequency on growth, feed conversion ratio and specific growth rate of red Tilapia, Oreochromis Sp
Biography:
Esther Michelle has completed her Masters in Aquaculture majoring in Aquaculture Nutrition at the age of 26 years from Universiti Malaysia Sabah. She is currently an educator in a technical institute teaching mainly on aquaculture feed and also fish husbandry
Abstract:
A preliminary experiment was carried out to investigate the effect of different feeding frequency on the growth, feed conversion ratio(FCR) and specific growth rate (SGR) of red tilapia, Oreochromis sp. Feeding twice a day (TW) was applied as a control in this trial compared with feeding once a day (ON) and thrice a day (TH) at a common hours of fish feeding. Initial average weight of fish used was 2.7±2 g. Trial was done in triplicates and 150 fish was randomly distributed into each aquarium. Experimental fish was fed using a commercial pellet based on 5% of total body weight per day. Final weight of fish was significantly higher in the ON treatment compared to TW and TH with 10.98 g, 8.29 g and 7.63 g, respectively. Fish weight was constantly increasing in all treatments throughout the experimental period. SGR for ON was 2g/day which means fish gain weight of 2g every day until the final day of the experiment. The present study shows that feeding once a day (ON) resulted to higher feed efficiency of feed based on the growth (linear relationship).
Amany Kenawy
National Research Centre, Eygpt
Title: Disease causing organisms in Procambarus clarkii and Gambusia affinis with emphasis on their role in biomonitoring of aquatic pollution
Biography:
Amany Kenawy is currently proffesor in National Research Centre, Eygpt
Abstract:
The health status of red swamp crayfish, Procambarus clarkii and mosquito fish, Gambusia affinis collected from Elmansoria canal, Giza, Egypt was investigated. The canal is known to receive lofty loads of pollutants from diverse anthropogenic sources. 113 bacterial isolates were obtained from the investigated fish specimens. Isolates were phenotypically identified as; Aeromonas hydrophila 26.54%, Vibrio parahaemolyticus 21.23%, Pseudomonas fluorescens 14.15%, E. coli 10.61%, Citrobacter spp. 7.96%, Enterobacter sp. 8.84%, Staphylococcus sp. 4.42% and Micrococcus sp. 6.19%. High gill infestations with Centrocestus sp encysted metacercariae were noticed in mosquito fish. No parasitic infestations were recorded in crayfish. Challenge experiment confirmed the pathogenicity of Aeromonas hydrophila isolates. The water analysis revealed high heavy metals levels with values; Ni 0.71, Pb 0.34, and Cd 0.2 ppm while Zn and Cu were in normal values. Metals analysis in crayfish and mosquito fish tissues denoted bioaccumulation. Crayfish muscles showed; Ni >Zn >Cu >Pb >Cd while their levels in mosquito fish demonstrated; Ni >Zn> Pb> Cd> Cu. Proliferative, degenerative and necrotic alterations were evident in histological sections. Results suggest that both crayfish and mosquito fish can serve as carriers for some fish disease pathogenic agents and a convenient tool for biomonitoring aquatic pollution
Wansika Kiatpathomchai
National Center for Genetic Engineering and Biotechnology, Thailand
Title: Development of DNA detection platforms using loop-mediated isothermal amplification to detect pathogens of aquatic animals
Biography:
Wansika Kiatpathomchai is currently a principal researcher and head of Bioengineering and sensing technology laboratory at BIOTEC Thailand. She has published more than 40 refereed journal papers and filed more than 20 Thai patents and petty patents. She received several of distinguished research awards such as L’Oreal for Women in Science Fellowship in 2005, “The Best research work” and Good invention award” from the National Research Council of Thailand in 2008 and 2012, “Gold Prize Awards” from Seoul International Invention Fair in 2012 and 2013, and “Taguchi Prize for Outstanding Research Achievement in Biotechnology” in 2013
Abstract:
An accelerated, loop-mediated isothermal amplification (LAMP) platform has been developed for sensitive and specific detection of few copies of target nucleic acids of pathogens. It operates under isothermal conditions by self-recurring strand-displacement DNA synthesis using specially designed primer sets. Although LAMP assays have been widely used in animal and human disease diagnosis, detection of positive LAMP reaction was conventionally visualized by agarose gel electrophoresis of amplicons with time consuming detection step. This can be avoided by replacement with cheaper, equally sensitive, more rapid and simpler methods including the use of a real time turbidimeter, use of lateral flow dipsticks or oligonucleotide-labled nanogold probes and use of metal indicators. Since these LAMP platforms do not require sophisticated equipment, they are more applicable for small or field laboratories. The time required for these integrated LAMP platforms is approximately 70 min (10 min for DNA/RNA preparation, 30-45 min for the LAMP reaction and 10-15 min for detection of the positive LAMP result). These platforms have been successfully applied for detection of shrimp pathogens (i.e., white spot syndrome virus, yellow head virus, Taura syndrome virus, Laem Singh slow growth virus, microsporidia and acute hepatopancreatic necrosis disease) and fish (tilapia) pathogens (Streptococcus iniae, S. agalactiae, Flavobacterium columnare, Francisella noatunensis subsp. orientalis, Aeromonas hydrophila, Shewanella putrefaciens and Infectious Spleen and Kidney Necrosis Virus). These integrated LAMP platforms are sensitive, simple, rapid and cost-effective and can applied in routine detection and are also more suitable for field conditions making them more suitable for use in quarantine/surveillance programs
Lenimfa P Molina
Cagayan State University, Philippines
Title: Documentation and inventory of aramang stocks N. tenuipes in northern Cagayan, Phillipines
Biography:
Lenimfa P Molina is currently working in Cagayan State University, Philippines
Abstract:
This study utilized the soft shelled red shrimps, aramang (Nematopalaemon tenuipes) in the preparation of aramang powder. The product could be used in the preparation of other valuable products and it could serve as a condiment. The study generally aimed to process and standardize the method in the preparation of aramang powder. It specifically aimed to determine the sensory qualities of the processed aramang powder using three (3) cooking methods, determine the consumer’s acceptability, its return on investment and its nutrifacts. Sensory assessment of the product was done from 0 – day to 30 – days at weekly interval. Results revealed no significant differences on appearance, odor, and general acceptability of the aramang powder using the three (3) cooking methods. Result of nutrition facts done at the Department Of Science and Technology Regional Office – Tuguegarao City revealed the following nutrient contents of the aramang powder: moisture 13.57%, ash 11.17%, fat 0.05%, protein 68.87%, carbohydrates 6.34% and sodium 296.61 mg/100 g. This reveals that the nutrients present in aramang powder is excellent and good for consumption as food. The production of aramang powder was found profitable with an ROI value of 83.29% and the level of acceptability was evaluated as “very much liked”.
Biography:
ESTHER has completed her Masters at the age of 26 years from Universiti Malaysia Sabah. He is currently an educator in Polytechnic of Sandakan Sabah Malaysia, majoring in Aquaculture Nutrition.
Abstract:
A feeding trial was carried out to evaluate the possibility of replacing fish meal with poultry by-product meal (PBM) at high inclusion levels (50-100%) in the formulated feeds of tiger grouper, Epinephelus fuscoguttatus. Fish meal-based feed (PBM0) served as the control feed and three PBM-based feeds with inclusion levels of 50 (PBM50), 75 (PBM75), and 100% (PBM100) were fed to triplicate groups of fish with mean body weight of 26.2±0.2 g. All formulated feeds were isoproteic (50%) and isolipidic (13%). Weight gain of juveniles ranged from 233 to 338% at the end of feeding trial. Final weight (g), weight gain (%) and specific growth rate (% day-1) of fish fed PBM0 were lower than other fish groups. The feed conversion ratio ranged from 1.1 (PBM50) to 2.0 (PBM0) with no significant difference detected in all treatments. Apparent digestibility coefficients (ADCs) were influenced by the inclusion of PBM in the feeds, with PBM50 recording better values in all measured ADCs. Meanwhile, replacement of fish meal with PBM has little influence on the whole body proximate compositions and body indices. The present study shows that PBM is an excellent alternative protein source for farming the tiger grouper juveniles with fish meal protein replacement level of 50% resulted in the best overall performances. Keywords: Alternative ingredients; Epinephelus fuscoguttatus; fish meal replacement; marine fish farming; poultry byproduct meal; tiger grouper
Khosrow Aein Jamshid
Iranian Fisheries Science Research Institute, Iran
Title: Iran shrimp industry strategic planning, Vision 2025
Biography:
Khosrow Aein Jamshid is an Expert in Ecology Department of Iran Fisheries Research Organization, Head of Abiotic Devision in Ecology Department of NSRI and also Head of National Shrimp Research Institute of Iran
Abstract:
Iran shrimp industry strategic planning, vision 2025 provided by Iran Shrimp Research Institute with contribution of experts of Iran Fisheries Organization, Iran Veterinary Organization, the universities, unions of fishermen, shrimp farming and exporter. The plan was approved by the Ministry of Jehade-e Agriculture of Ir. Iran. Objectives, policies, strategies and action plans of shrimp industry has been prepared for 2010 to 2024. Changing the short-term attitudes and behaviors of policy makers to the long term benefits, changing the fishing management policies to right-based and ecosystem-based management, improve the sustainability of fisheries instead of short-term employment of fishermen, implementation, monitoring and updating technical guidelines for responsible fisheries, standardization of improvement of fishing technology to promote selective fishing and improvement the performances of fishing methods, conservation of nursery grounds and sensitive fish habitats, development of artificial reefs, mitigation of environmental pollutants, improve the knowledge level of fisheries stakeholders can reduce the pressure on marine resources and lead to the sustainable exploitation of marine resources. Although that the shrimp sales and marketing is the last chain loop of farmed shrimp industry but it should be considered as the most important and its core problem. The current world economic situation, hard competition in the international markets, lead us to attain the look on the domestic market as the important way for sustainability of Iran shrimp industry development.
Mojgan Khodadadi
Islamic Azad University, Iran
Title: Comparison of omega6 fatty acids amount in male and female of Otolithesruberin Abadan and Khorramshahrs coated water
Biography:
Mojgan Khodadadi has completed his PhD at the age of 33 years from Islamic Azad University, Tehran Branch. He is the Supervisor of Zynabe Bavi, a Master Student of Aquaculture. He had published more than 50 papers in reputed journals (ISI and ISC) and has been serving as an editorial board member of repute. He is managing editor in Marine Biology Iran and Ecobiology of Wetland.
Abstract:
Otolithes ruber with rather fusiform body and silver colour, has an appearance that customers like it.In order to implementation this study 45 Otolithes ruber from Abadan and Khoramshahr costal water in spawning season since December of 2013 to February 2014 were gathered randomly. After opening stomach and removing gonads and then evaluation and sexual maturation stage determination by 5 stages key (Biswas, 1997) were placed in spawning, peak of sexual maturation and after spawning. Results showed that in female species omega6 fatty acid in 3 stages including before sexual maturation (3.88± 0.96), peak of sexual maturation (6.53± 1.02) and after spawning (8.15 ± 1.03) had significant difference at 5% level maximum and minimum of fatty acid amount were related to after spawning (8.15± 1.03) and before sexual maturation (3.88± 0.96), respectively. In male Species Omega6 fatty acid has significant different in 3 stages including before sexual maturation (3.33± 0.86), peak of sexual maturation (4.93± 1.43) and after spawning (7.66± 1.44) at 5% level. Maximum and minimum of fatty acid amount were related to after spawning and before sexual maturation stages, respectively. Reproductive processing female fish, nutrition transform to oocytes in ovary during making yolk to zygot and stored there to required nutrition for zygot and newborn larva that should prepare by parents. Activity increase in fishes in sexual maturation and more energy was spent for reproductive activities and sexual maturation. Because of this storage energy supply in fish must be more that can use more energy and compensate used energy.
Sharif Rohani M
Iranian Fisheries Science Research Institute, Iran
Title: “AVISHITâ€, a new herbal drug for substitution of Malachite green
Biography:
Sharif Rohani M is currently working in Iranian Fisheries Science Research Institute, Iran
Abstract:
Fungal pollution have induced high economic losses specially in trout hatchery. Malachite green is the most effective chemical for treatment and profilaxy of fungal disease but it has many disorders for environment and public health. herbal drug like “AVHSHIT” derived from Zataria multiflora ( Avishan –e- shirazi ) have good effects for aquatic fungal prevention and control. About 16 kg trout eggs from 3-4 years old Oncorhynchus mykiss brooders were evaluated to assess the capability of “AVISHIT” in control of fungal pollution. During 2 years in 9 stages, the effects of 6 doses of this essence (1,5,10,25,50 and 100 ppm ) was evaluated daily with 3 repetition until the eyed egg stage(about 18-20 days).Besides , under the equal condition ,the positive control group was treated with 1 ppm malachite green and the negative control group assessed without any drugs. Physico and chemical parameters like pH, Temperature and dissolved oxygen determined during this survey .MIC (Minimum Inhibitory Concentration) technic is done for In vitro assay fungicide potentially. In GP agar media , Saprolegnia parasitica and Fusarium solani isolated from fungal eggs. MIC result of “ AVISHIT “ against Saprolegnia was 0.9 and against Fusarium was 1.6 ppm. the measure of water physical and chemical factors (before and after treatment ) weren’t significant. “ AVISHIT “ specially in the doses of 25 ,50 and 100 ppm had significance difference with negative control group ( p< 0.05 ) and created suitable inhibition against water fungi. The results of this survey covered Marking etal. (1994) suggestions about characterisation of fungicide drugs. High safety of “ AVISHIT ‘ for environment and public health , friendly usage and cost benefit are better indications of this agent compare with malachite green .
Kiuomars Rohani Ghadikolaei
Ecological Research Institute, Iran
Title: Water quality and sediment conditions in white shrimp Litopenaeus vannamei (boone) pond during the cultivation period in Iran
Biography:
Kiuomars Rohani-Ghadikolaei has completed his PhD at the age of 42 years from University Science Malaysia (USM), Malaysia. He is the head of Aquaculture Department of Persian Gulf and Oman Sea Ecological Research Institute, Bandar Abbass, Iran. He has published more than 3 papers in reputed journals and has been serving as an editorial board member of Iranian Fisheries Research Journals
Abstract:
Considering shrimp farming as a new and improving aquaculture practice in southern coastal parts of Iran, it faces some critical problems in terms of water quality that need to be monitored. The present study purposed to assess the water quality and sediment conditions in L. Vannamei ponds during the shrimp farming in Teyab, Hormozgan, Iran. The results indicated that during the culturing period, mean values for temperature, salinity, pH and dissolved oxygen were not significantly different in 3 farms, whereas the levels for Chlorophyll-a and total organic matter (TOM) showed the highest content onwards to the end of culturing period. The average total length or final weigh showed no significant differences in 3 farms at the end of culturing period. Positive correlation was found between pH and water temperature, pH and dissolve oxygen (DO), pH and ammonia; Chl-a and DO; growth parameters and DO, growth parameters and Chl-a. However a negative correlation was observed between TOM and pH, TOM and water temperature, TOM and ammonia, TOM and pH; Chl-a and water temperature, Chl-a and salinity, Chl-a and transparency; growth parameters and salinity, growth parameters and pH, growth parameters and ammonia. Dendrogram of temporal clustering showed that the water quality was relatively consistent during early culturing period and then represent an unstable condition from the second month towards end of culturing period. However, water quality parameters should be monitored to serve as guide for managing a pond so that conditions that can adversely affect the growth of prawns can be avoided
Godwin N. Isitor
Rivers State University of Science and Technology, Nigeria
Title: Veterinary perspectives on conservation of indigenous freshwater ornamental and food fish biodiversity through aquaria systems
Biography:
Godwin N. Isitor completed his Ph.D at the age of 31 years from Ahmadu Bello University, Zaria, Nigeria. He is presently a Professor at the Department of Animal and Environmental Biology. He had a successful stint at the School of Veterinary Medicine, The University of the West Indies, Trinidad and Tobago Campus between 1986 and 2009, where he rose to the rank of Professor of Veterinary and Microscopic Anatomy in 2004 prior to returning to Nigeria. He has over 50 schorlarly publications in refereed journals, and he is Currently leading a team of researchers in implementing a TETFUND sponsored research project titled “Conservation of ornamental and food fish biodiversity through aquaria systems”.
Abstract:
An on-going research project on conservation of ornamental and food fish biodiversity through aquaria systems is presented. Both indigenous and exotic ornamental fish, as well as a few food fish, are aimed at being conserved under the proposed project. The overall rationale is that of utilizing a novel procedure, involving aquaria systems, to collect, propagate and monitor the different species being conserved, and at same time build selected stocks that can be readily disseminated to commercial outfits which in turn are expected to supply products to the end user consumers. The aquaria mix ranged from simple rectangular glass constructs to more complex circular plastic types of a maximum of 150L water holding capacity; some of which are fitted with vital accessories, such as aerators, aquaria lights, artificial and natural plants, as well as water flow-through input and output taps. The conserved fish-types are mostly endangered freshwater species of ray and fin-fish, e.g. gobies, cichliids, polypteriids, clariids, locariids, notopteriids, and anabantiids, as well as reptiles and crustaceans (terappins and decapods). In the conservation process, veterinary challenges, in particular, helminth infestations and fungal infections appear to be an important issue in the overall success of the exercise. The modalities for administration of ivermectin in the aquaculture and its subsequent use for effective control of helminthiasis are being investigated towards achieving the desired goal of effective conservation, adaptation and propagation of the species through aquaria system
Osineye Oluseun Motunrayo
Federal College of Fisheries and Marine Technology, Nigeria
Title: Comparative effects of different sources of protein on the performance of Clarias gariepinus
Biography:
Osineye Oluseun Motunrayo has a B.Sc (Hons), M.Sc. and Ph.D in Agricultural Biochemistry and Nutrition of University of Ibadan, Nigeria. He has been teaching Nutritional biochemistry, General Biochemistry, Fish Nutrition, Diet and Feed Ingredients’ evaluation, Principles of Agriculture, Monogastric and Ruminant animals’ nutrition, in University and Polytechnic systems in Nigeria. He has been relevant and contributing his quota to the development and promotion of activities and functions in animal agriculture, biochemistry, human nutrition, capture fisheries and aquaculture, garnished with adequate knowledge of research methodology.He has published two textbooks and over 20 journal articles. He is a former Deputy Provost (Academic) of the Federal College of Fisheries and Marine Technology, Lagos
Abstract:
Four hundred and eighty (480) fingerlings of Clarias gariepinus were randomly allotted to seven isocaloric and isonitrogeneous dietary groups, replicated thrice in a completely randomized design, in an 8-week feeding trial. Parameters measured are dry matter intake, weight gain, feed conversion ratio, digestibility, protein efficiency ratio and specific growth rate.The results revealed that, although the efficiency of coppens in all the parameters measured was significantly (p<0.05) higher than any of the other diets, but the values recorded for diets 4 and 5 (with Danish fishmeal and Soybean meal + Methionine as protein sources respectively), were comparable to values recorded for coppens, as follows: Coppens, Local fishmeal, Maize + Soybean meal, Danish fishmeal, Soybean + Methionine, Blood meal, Chicken Intestine meal and Chicken feather meal respectively recorded 16.98, 8.27, 6.99, 12.61, 11.92, 6.97, 5.95 & 4.20, for Mean Weight Gain(g); 3.45, 2.67, 2.81, 3.08, 3.33, 2.75, 2.20 & 2.12, for Specific Growth Rate; 1.78, 3.16, 3.46, 2.60, 2.78, 3.82, 3.96 & 4.20, for Feed Conversion Ratio; 42.57, 30.07, 35.08, 41.62, 36.96, 35.82, 33.37 & 32.93, for Protein Efficiency Ratio; 30.22, 26.13, 24.19, 32.79, 33.14, 26.63, 23.56 & 17.20, for Dry Matter Intake(g); 90.4, 69.4, 81.8, 74.4, 66.8, 60.7 & 34.3, for digestibility(%); 700, 141,127,219,395,93,88 & 88, for cost of 2kg feed (N:k). Soybean meal+ Methionine, as protein source has been shown in this study to be most profitable when the cost is considered.
Jemimah Timothy Ekanem
AkwaIbom State University, Nigeria
Title: Perceived impact of climate change on fish production by fish farmers in AkwaIbom State, Nigeria
Biography:
Jemimah Timothy Ekanem completed her Ph.D in 2013 from Michael Okpara University of Agriculture, Umudike, Umuahia, Abia State, Nigeria. She is currently a Lecturer at AkwaIbom State University, (AKSU), Uyo, Nigeria. She is an Agricultural Extension Communicator and a Rural Developer. She has taught in institutions of higher learning for over 10 years, has more than 20 publications in revered Journals, both International and Local and attended many conferences.
Abstract:
The study was conducted in AkwaIbom State, the South-South geopolitical region of Nigeria. It aimed at examining the sources/level of awareness, the perceived impact of climate change on fish production and possible mitigation or adaptation measures taken by Fish Farmers in the study area. 120 Fish Farmers were selected using a multistage sampling method and data were collected through semi-structured questionnaire. Data were analysed using descriptive and inferential statistics. From the findings, it was evident that farmers have heard about climate change but had low level of knowledge of the impact of climate change on their production capacity as their main sources of information were from their fellow farmers and personal experience. High rainfall, variability in temperature, massive flood, poor harvest and food insecurity and hunger, were some of the perceived impact of climate change on fish production and measures like adjustment of stocking intervals, stocking of quick maturing/adaptive species, increased use of concrete ponds by the fish farmers in the study area. The study concluded that there was need for increased awareness and intensive education of Fish Farmers by Extension Workers through the use of individual, group and mass media contacts on the effects of climate change on fish production and perhaps, unveil some indigenous measures which could be employed in mitigating climate change effect on fish production to the Farmers.
- Young Researcher Forum
Session Introduction
Carole Blay
Ifremer, France
Title: Combined interaction of size phenotype of donor and recipient pearl oyster and gene expression on final pearl growth in Pinctada margaritifera
Biography:
Carole Blay has graduated as biology engineer in 2009 (France). She has worked on the domestication of cultivated crops such as Pennisetum glaucum and Digittaria sp. of the research institute for the development. Then, she has worked for IFREMER on the “pearl oyster” Pinctada Margaritifera on the genetic improvement of the donor oyster. In 2014, she has started a phD on genetic determinism of pearl quality trait on pearl oyster Pinctada margaritifera. This work will improve the knowledge on the effects of genotype and research genes responsible for the expression of the quality traits (color and pearl size)
Abstract:
The pearl oyster, Pinctada margaritifera, produces valuable cultured pearls as a result of the biomineralization process of a selected mantle graft from a donor oyster inserted together with a nucleus into the gonad of a recipient oyster. Production and annual exportation of cultured pearls increased from 86 kg in 1980 to 16 tons in 2012 valuing 65 million Euros of incomes. The commercial value of cultured pearls depends on different parameters, including pearl size, shape, colour, darkness level, classification grade, lustre and surface quality. Overall feature, pearl size is the most valuable one, with the largest pearls being the most valuable. To explore the respective roles of donor and recipient in pearl formation, a uniform experimental graft was designed with large and small donor oyster shell phenotypes, used to graft recipient oysters monitored for their growth-related traits every 3 months over one year period. At the same time, phenotypic parameters corresponding to pearl size and quality traits were recorded. Phenotypic interaction analysis demonstrated 1) a positive correlation between recipient shell biometric parameters and pearl size, 2) donor effect on cultured pearl quality traits. Furthermore, the expressions of biomineralization biomarkers encoding proteins in the aragonite or prismatic layer showed: 1) overexpression of an aragonite-related gene in the large-shelled donor phenotype in the graft tissue, and 2) correlation of gene expression in the pearl sac tissue with pearl quality traits and recipient biometric parameters. These results emphasize that the recipient mainly drives pearl size and the donor mainly drives pearl quality traits.
Kay Lubke
Bavarian States Research Center for Agriculture, Germany
Title: Stress and fish welfare - Non-invasive measurement of Cortisolin fish holding water
Biography:
Kay Lubke has completed his studies on marine and fish biology at Rostock University (Diploma) in 2013. He is now a scientist at the Institute for Fisheries of the Bavarian States Research Center
Abstract:
Today the issue of animal welfare is of increasing importance, especially in farmed fish. Both, consumers and producers, are highly interested in a good welfare status of the fish for ethical, as well as product quality reasons. In this context some management actions applied in aquaculture, e. g. high stocking densities or raising fish in artificial habitats, came under criticism. To evaluate common husbandry actions in aquaculture a non-invasive method for measuring cortisol, the most important stress parameter, in fish holding water was used. A set of investigations was done manly with rainbow trout. In the beginning tests were made to gain information about cortisol release under normal husbandry conditions (reference/basic values). First results indicated a basic release rate of approximately 0.1 ng/(g*h) for smaller trout and even lower values for larger fish sizes. Furthermore, the short term influences of management actions were studied as well as the long term influence of different stocking densities. To determine the welfare in the long term study, further parameters as the general performance of the fish, e. g. growth, food conversion, survival, and the fin condition index was used next to the cortisol release. Here we found that actions like netting and transferring fish to another pond provoked a sharp increase in cortisol release rate, but only for one to two hours. Furthermore, high density does not lead to increased cortisol release or impaired welfare in other parameters, if environmental conditions are managed correctly
Fabio Cervellione
Skretting Aquaculture Research Centre, Norway
Title: Effect of starvation on hepatopancreas of whiteleg shrimp (Penaeus vannamei) using computer-assisted image analysis on paraffin and frozen sections
Biography:
Fabio Cervellione graduated as DVM at Milan University, and completed his MSc in Aquatic Veterinary Studies at Stirling University. He worked for 9 years as a diagnostic veterinarian for fresh water farmed species for Skretting, which is a leading shrimp and fish feed company in the aquaculture sector. He works now for Skretting Aquaculture Research Centre in Norway, in the Health Department, directed by Dr. Charles McGurk. He is a PhD candidate at Ghent University, focusing on semi-quantitative histology of the gastro intestinal tract of whiteleg shrimp (Penaeus vannamei) with Prof. Wim Van den Broeck, Professor of Cell Biology and Histology
Abstract:
The hepatopancreas is the main organ of the gastro-intestinal tract in crustaceans and it is routinely assessed by pathologists for signs of disease and health monitoring because it is the site of digestion, nutrient absorption, reserve storage, detoxification, synthesis and secretion of digestive enzymes. The hepatopancreas is mainly composed of 4 different cell types: E-cells, B-cells, F-cells and R-cells. R-cells resemble the absorptive cells of the vertebrate intestine and store mainly lipids and glycogen. Many decapods can survive weeks or even months of total starvation. In the past, a few studies focused on the influence of feeding on hepatopancreas structure but none of them using computed-assisted image analysis. Image analysis is a fast, objective and applicable method for routine screening of high number of samples, both for diagnostic perspectives and research applications. In the present study, whiteleg shrimp (2±1g, C inter-moult stage) were housed individually in glass tanks (27±1°C, pH at 7.8-8.1, and salinity at 20±1gL-1). Three feeding regimes were compared over a 15 days period: fed (5% of body weight/day); starved; and re-fed after 10 days of starvation. Morphological changes caused by starvation were analysed in paraffin sections and frozen sections with computer-assisted image analysis software (Visiopharm®). Hepatopancreatic parameters measured were: total tissue area, lumen area, lipid droplets, F-cells, and infiltration of haemocytes. Effect of starvation on the ultrastructure of the hepatopancreas was also studied by TEM. Application of image analysis on a routine basis will permit health monitoring of the nutritional status in farmed decapods
Yishuai Du
Chinese Academy of Sciences, China
Title: Development and application of a real-time PCR assay for the detection of Aeromonas salmonicida
Biography:
Yishuai Du has completed his PhD at the age of 28 years from Institute of Oceanology, Chinese Academy of Sciences(IOCAS). He is the assitant professor of IOCAS. He has published 3 papers in the field of the disease control of marine animals
Abstract:
A rapid, economical, specific and sensitive real-time polymerase chain reaction (RT-PCR) assay coupled with SYBR Green I chemistry was developed for the quantitative detection of Aeromonas salmonicida (A. salmonicida) isolated from farmed Atlantic salmon (Salmo salar) with the symptoms of furunculosis. The set of primers designed from the virulence array protein (vapA) gene was specific to the A. salmonicida and didn’t cross-react with other bacteria. Compared with the conventional PCR, RT-PCR had a lower detection limit of 5.6 copies of the positive plasmids. The standard curve, which showed the relationship between the copies of A. salmonicida and its cycle threshold (CT) value, could be described as: log (copies of A. salmonicida) = -0.3213 CT + 10.721. The quantitative detection of copies of A. salmonicida in different tissues of the moribund Atlantic salmon showed that A. salmonicida could be detected in all tissues detected; the spleen contained the largest number of A. salmonicida, and then the kidney. These results suggest that the RT-PCR assay reported here is a specific, sensitive and quantitative method for detecting A. salmonicida in different tissues of Atlantic salmon. It can be used for the routine tests of A. salmonicida in the local aquaculture enterprise and for the research of infection routes of A. salmonicida to Atlantic salmon
Biography:
Arpita Dalal is pursuing her PhD under the supervision of Dr.Susmita Gupta (Associate Professor) of the department of Ecology and Environmental Science, Assam University, Silchar, India and is a senior research fellow (SRF) of the Department of Science and Technology (DST), New Delhi, India. She has cleared National Eligibility Test (NET), December 2014 for Environmental Sciences and recently she has been awarded and designated as JSPS HOPE Fellow at the 8th HOPE Meeting with Nobel Laureate, JSPS, Japan. Currently she has been selected in a PhD exchange programme jointly sponsored by the Department of Biotechnology (DBT), New Delhi, India and British Council, UK under the programme ‘Newton Bhabha PhD placement programme’, and isnow working as a visiting research associate at Queen’s University, Belfast, UK under the guidance of Prof. Jaimie Dick for a period of six months. At present she has three papers in peer reviewed journals (SCI indexed)
Abstract:
Aquatic insects are commonly known as good fish diet, bioindicators of freshwater pollution and can be utilised asbiocontrol agents. Understanding the drivers of insect distribution, abundance and species richness is thus vital for healthy fish stocks and aquaculture practices. The present study examined spatial and temporal variation in aquatic insect assemblages in several different lentic (standing freshwater) ecosystems- oxbow lake, Floodplain Lake, rural and urban ponds, and two agricultural fields of Cachar district, Assam. With kick sampling, aquatic insects were collected along with water samples in four seasons (post monsoon, winter, premonsoon and monsoon) during 2013-14. We collected2260 individuals belonging to 119 taxa,36 familiesand8 orders. The highest number oftaxa was recorded from the floodplain lake,with thelowest from the oxbow lake. Post monsoon and winter recorded highest taxa, whereas monsoon had the lowest. Engelmann’s scale of dominance showed Anisops breddini (Water boatman), Cloeon sp. (Mayfly), Micronectas cultellaris (Lesser water boatman), M. Haliploides (Lesser water boatman), and Ochthebius sp. (Minute moss beetle) as the eudominant taxa from the different lentic systems. There was a significant positive correlation ofbothtaxa richness and taxa density with dissolved oxygen and potassium, whereas there were negative correlations with water temperature, rainfall, water depth and size of water bodies. In addition, we discuss here the factors regulating the distribution of aquatic insects in different seasons.
Biography:
Mahammed Moniruzzaman is a post-doctoral research fellow in Fish Endocrinology Laboratory, University of Calcutta, Kolkata, India. He has completed his PhD from Environment Endocrinology Laboratory, Visva-Bharati University, Santiniketan, India and currently working on to investigate whether phytosterols can protect ovarian and liver tissue against lipid peroxidation, bacterial infection at the same time can alter gender specific immunostimulation
Abstract:
Present communication is an attempt to demonstrate the influence of phytochemicals on the action of maturation inducing hormone (MIH) on the maturation of oocytes. The oocytes from gravid female catfish Mystus dibrugarensis were isolated and incubated separately in Medium (a) MIH (1 µg/ml), (b) ethanolic extract of Tribulus, (c) Aqueous extract of Tribulus (d) MIH administered with (i) ethanolic extract of Tribulus, (ii) Aqueous extract of Tribulus and the effects of treatment on oocyte maturation were evaluated by considering the rate (%) of germinal vesicle breakdown (GVBD). Incubation of oocytes in medium containing only Tribulus extract (both aqueous and ethanolic) did not result in any significant change in GVBD percentage. Nearly all the oocytes underwent GVBD when incubated with MIH for around 20h. However, it was quite interesting to observe that incubation of oocytes with Tribulus extract co-administered with MIH in the medium, led to an accelerated rate of GVBD in the oocytes. Further study revealed that incubation with Tribulus extract accelerates the action of MIH on the formation of a complex of two proteins (MPF), a regulatory component called cyclin B and the catalytic component protein kinase known as cyclin-dependent kinase, Cdk1. Notably, administration of Tribulus extract, led to a sharp reduction in the follicular level of MDA (malondialdehyde) - an intra-cellular stress marker, and significant increase in the activity/level of both enzymatic [SOD (superoxide dismutase), CAT (catalase), GST (glutathione transferase), GPx (glutathione peroxidase) and GRd (glutathione reductase)] and non-enzymatic [GSH (reduced glutathione)] antioxidants. Collectively, current study presents the evidence of phytochemical administration in the incubation medium alleviates oxidative stress of pre-ovulatory follicles by stimulating different antioxidants and ameliorates MIH actions on the process of final oocyte maturation through formation of MPF
Biography:
Abstract:
Argulosis is known to cause large scale mortality of fish in various parts of the world. Although different control measures have been adopted against this disease, but none of those are found to be full proof and parasitaemic recurrence is an obvious because of non incorporation of the parameters of life cycle and development into the management protocol. While investigating embryonic development of the parasite of Argulus bengalensis an interesting phenomenon came to our notice that the egg stage of the parasite is intimately associated with an another organism. Under both laboratory & field conditions it has been observed that a day after egg laying, the rotifer Philodina roseola colonize and exhibit active feeding on the egg jelly. Mechanical removal of the rotifers from the egg strips resulted in complete (100%) failure in hatching inspite of full term embryonic development, while the eggs in rotifer infested condition hatched successfully. The present study thus approves a service-resource mutualistic relationship between A. bengalensis and P. roseola. In this relationship the rotifer renders service to the parasite through removing solidified jellly coat and getting nutritional benefit from it. It is proved that this mutualism is obligatory to Argulus but facultative to the rotifer. In corroboration with the relationship of Philodina spp. with other species the evolution of mutualism between these two organism is apprehended as a transformation of a parasitic relationship into mutualism. An intervention in this relationship may offer a definite clue for management of this parasitic infestation
Ryan S. Mohammed
The University of the West Indies, Trinidad and Tobago
Title: Parasite-host interactions at elevated temperatures- Getting into hot water: Sick guppies frequent warmer thermal conditions
Biography:
Ryan S. Mohammed is currently completing his PhD in aquatic parasite and host dynamic at elevated temperature at The University of the West Indies. He is also the President of the Aquaculture Association of Trinidad and Tobago and the chairman of the Tilapia Task Force under the Ministry of Agriculture, Land and Fisheries. His main research interest lies in aquatic ecology and aquaculture and has published in both fields
Abstract:
One of the major predicted effects of climate change within fifty years is a 4 - 6°C projected increase of ambient temperature. Many of the effect of these increases to tropical freshwater ecosystems are still unknown. One particular importance is the interaction of ecto-parasites and their ichthyofaunal host during this increase in temperature. This project proposes a series of experiments at elevated temperatures investigating fish behavior on a model species, the Trinidadian guppy (Poecilia reticulata), parasite tolerance, parasite interactions and parasite transmissions (Gyrodactylus turnbulli). A choice chamber experiment was used to investigate the thermal preferences of P. reticulata, when infected with a common helminth ectoparasite G. turnbulli, in female-only and mixed-sex shoals. The temperature tolerance of G. turnbulli was also investigated by monitoring parasite population trajectories on guppies maintained at a continuous 18, 24 or 32oC. Regardless of shoal composition, infected fish frequented the 32oC choice chamber more often than when uninfected, significantly increasing their mean temperature preference. Parasites maintained continuously at 32oC decreased to extinction within three days, whereas mean parasite abundance increased on hosts incubated at 18 and 24oC. We show for the first time that gyrodactylid-infected fish have a preference for warmer waters and speculate that sick fish exploit the upper thermal tolerances of their parasites to self medicate. The implications of this on tank based aquaculture will be discussed as a potential mechanism for extoparasite control on fish
Biography:
Ana Mansourkiaei completed her PhD at the age of 34 years from Islamic Azad University Scientific and Research Branch. She is a diving instructor. Also she had taken her diving certificate from CMAS. She had published more than 5 papers in reputed journals.
Abstract:
Narrow-barred Spanish mackerel (Scomberomorus commerson) and Long tail tuna (Thunnus tonggol) are epipelagic and migratory species of family Scombridea which have a significant role in terms of ecology and fishery. Phylogenetic relationships of S. commerson from the Persian Gulf and Oman Sea and Arabian Sea, and T. tonggol from Oman Sea were investigated through sequence data of mitochondrial DNA D-loop region using High Resolution Melting-Real Time PCR. S. commerson were classified into 20 groups and T. tonggol were banded into 8 groups. Finally 40 samples of S. commerson and 16 samples of T. tonggol were chosen to sequence for phylogenetic and genetic analyses. None clustered NJ tree indicated the proximity amid S. commerson in four stations and T. tonggol into stations. As figures demonstrated in sequence analyses of mitochondrial DNA D-Loop region a sublimely high degree of genetic similarity among S. commerson from the Persian Gulf and Oman Sea and Arabian Sea and also among T. tonggol from the Oman Sea were perceived, thereafter, having one stock structure of S. commerson in four regions and one stock structure of T. tonggol in two regions were proved. And this approximation can be merely justified by their emigration process along the coasts of Oman Sea and Persian Gulf and Arabian Sea Accordingly, The assessment of distribution patterns of 40 samples of S. commerson and 16 samples of T. tonggol in the constructed Phylogenetic tree and using mtDNA D-Loop sequences ascertained that no significant clustering according to the sampling sites were concluded. And Fst values shows this similarity as well.
Mohammad Gorgij Jaski
Bandar Abbass Azad University, Iran
Title: Culture of Spirulina platensis in different source of nitrogen for optimizing of protein content
Biography:
Mohammad Gorgij Jaski has completed his MSc. at the age of 30 from Azad University of Bandar Abbass, Iran and now is PhD student in Aquaculture. He is the head of Sontderaf Shrimp Production Company, Bandar Abbass, Iran. He has published more than 2 papers in reputed journals
Abstract:
The major nutritional interest in Spirulina is due to its high protein content, ease of digestion and a significant content of vitamins, minerals, amino acids and pigments. Nitrogen is known to have a strong influence on the metabolism of lipids and protein in various microalgae. In the present study, the production of S. platensis was optimized in terms of biomass and metabolites by using different nitrogen regimes. S. platensis was grown in Zarrouk’s medium in a 3000ml Erlenmeyer flask, in which the NaNO3 was replaced by NH4Cl, NH4NO3 and KNO3 with concentrations of 0.010, 0.025 and 0.050 M. Cultures were incubated at temperature of 30oC and initial pH of 9.5 under 12/12 hour light-dark photo period with normal white light. The results clearly showed that S. platensis successfully cultivated by using different nitrogen regimes and maximum biomass was produced in medium containing NH4NO3. The maximum protein content was obtained in culture containing NH4NO3 followed by NH4Cl and KNO3. Moreover, in all S. platensis cultures, increasing in nitrogen concentrations, led to an increase in maximum biomass and minimum protein content; whereas with increasing nitrogen concentrations, chlorophyll-a content increased more slowly and reached relatively high values only at very high nitrogen levels. However, chlorophyll-a content did not show any significant, when the nitrogen were varied in term of regimes and levels. Overall, the results of present study clearly showed that using NH4NO3 can be considered as a promising nitrogen source for S. platensis cultivation for achieving optimal biomass and protein production
- Workshop on Animal Welfare in Aquaculture
Session Introduction
Helmut Wedekind
Bavarian States Research Center for Agriculture, Germany
Title: Animal Welfare in Aquaculture
Biography:
Helmut Wedekind has completed his PhD at the age of thirty one years from Göttingen University on postdoctoral studies on intensive catfish farming. He is the director of the Institute for Fisheries, part of the Bavarian States Research Center for Agriculture in Starnberg, which is a research and education center for fisheries and aquaculture. He has published papers in reputed journals and in national fisheries and aquaculture journals. Moreover he is a lecturer at the Technical University of Munich and other German high schools in the field of aquaculture
Abstract:
Animal welfare is an actual and quite relevant topic in aquaculture. Facing serious concerns by consumers and critical groups, the industry is obliged to evaluate their practice in the varying farming systems. So far, the monitoring and safe guarding of fish welfare has received considerable attention by researchers in recent years. During aquaculture production several environmental conditions (e. g. water quality, illumination) and management procedures (e. g. feeding, stocking, handling) can be stressful for fish. Moreover, the social structure in holding facilities or other environmental stressors can lead to a depression in performance and can be detrimental to health. In this context it has to be pointed out that satisfying the physiological and behavioral needs of the organisms cultured, is in consistence with the producers interest. Only if the husbandry conditions are close to the optimum, maximum growth and health can be assured, which consequently result in best productivity. Actual research in fishes is focused the evaluation and measurement of parameters for the welfare status. Besides animal behavior and performance, measures of health restrictions and physiological stress reactions are indicative for the welfare status of farmed animals. Some specific indicators, such as health related parameters, are precisely indicative for a stressor (e. g. gas bubble disease). Others (e. g. thin or skin lesions) can have different causes or can generally indicate suboptimal husbandry conditions. Behavioral indicators are even less specified for aquaculture species. To distinguish stress induced behavioral and abnormalities, further research is required e.g. for the definition of normal behavior under culture conditions. For this, the development of assessment methods with a high level of practical suitability for farms, as well as monitoring tools for fish welfare, is a current challenge for researchers
- Workshop on Aquaculture Finance & Derivatives
Session Introduction
Christian Oliver Ewald
University of Glasgow, UK
Title: Aquaculture finance & derivatives
Biography:
Christian Oliver Ewald was Chair in Financial Economics and Head of Economics, Adam Smith Business School, University of Glasgow. He educated at the Universities of Mainz (MSc) and Heidelberg (PhD) and holds a higher doctorate (Habilitation) from the University of Kaiserslautern. Prior to moving to Glasgow in 2011, he has held positions at the University of Sydney, Nottingham University Business School (China), University College Cork, University of St. Andrews and the University of Leeds.