Aquaculture & Fisheries

Biography

Biography: Sharif Rohani M

Abstract

Fungal pollution have induced high economic losses  specially  in  trout  hatchery. Malachite green is the most effective chemical for treatment and  profilaxy of fungal disease but it has many disorders  for environment and public health. herbal drug  like “AVHSHIT” derived from  Zataria multiflora  ( Avishan –e- shirazi ) have good effects for aquatic fungal prevention and control. About 16 kg trout eggs from 3-4 years old Oncorhynchus mykiss brooders were evaluated to assess the capability of “AVISHIT” in control of fungal pollution. During 2 years in 9 stages, the effects of 6 doses  of this essence (1,5,10,25,50 and 100 ppm )  was evaluated daily with  3 repetition until the eyed egg stage(about 18-20 days).Besides , under the equal condition ,the positive control group was treated with 1 ppm malachite green and the negative control group assessed without any drugs. Physico and chemical parameters like pH, Temperature and dissolved oxygen determined during this survey .MIC (Minimum Inhibitory Concentration) technic is done for In vitro assay fungicide potentially.  In GP agar media , Saprolegnia parasitica and Fusarium solani isolated from fungal eggs. MIC result of “ AVISHIT “  against Saprolegnia was 0.9 and against Fusarium was 1.6 ppm. the measure of water physical and chemical factors (before and after treatment ) weren’t  significant. “ AVISHIT “ specially in the doses of 25 ,50 and 100 ppm had significance difference with negative control group ( p< 0.05 ) and created suitable inhibition against water fungi. The results of this survey covered Marking etal. (1994) suggestions about characterisation of fungicide drugs. High safety of  “ AVISHIT ‘ for environment and public health , friendly usage and cost benefit  are better indications of this agent  compare with malachite green .