Alejandro J Yanez Carcamo
Universidad Austral de Chile
Chile
Title: Novel media for the culture of the salmonid pathogen Piscirickettsia salmonis and their biotechnological uses in the salmon industry
Biography
Biography: Alejandro J Yanez Carcamo
Abstract
Piscirickettsia salmonis is a facultative intracellularGram-negative bacterium isolated from salmonids inChile and constitutes one of the main problemsin farmed salmonids and marine fish around the world. The use ofartificial media represents an alternative to diminish the costof cell lines and reducescontamination problems.Recently, we have developed the first broth media and two new solidblood-free media, termed Astral SRS-Broth and Austral-TSFe agar and Austral-TS Hemagar,. Thesenovel media have shown to beproper for the bacterial isolationand routine culture in thelaboratory. Moreover, the data obtained with AUSTRAL-SRS broth, indicate that this mediumcan be successfully used in susceptibility tests ofP. salmonis isolates and the CLSI accept and indicatethis media in the new guideline edition 2014.The in vitro drug susceptibility tests contribute to anunderstanding of the pharmacokinetic data in fishas well as evaluate the drug resistance of this pathogen. Indeed, the genome sequencing of a highly pathogenic strain AUS005, let us to study the expression levels of several multidrug resistance pumps.Finally, the purification immunogenic protein obtained from P. salmonis provides a suitableplatform to simplify the preparation of a new class oflow-cost vaccinethat shown high rate of protection. These new biotechnological tools will help salmon producers to take treatmentdecisions and control the disease. FONDAP-INCAR 15110027.
